منابع مشابه
A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins.
Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice. Tissues from these mice are commonly formalin fixed and paraffin embedded (FFPE) for histological studies. However, this results in inactivation of the natural fluorescence of these proteins, requiring their detection by immunolo...
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The chemistry of the reaction of 2-hydroxy&nitrobenzyl bromide (HNB-Br) with tryptophan ethyl ester to form monosubstitution adducts has been elucidated. The initial compounds are formed by addition at position 3 of the indole ring to form two diastereomeric indolenines. These compounds can then cyclize and rearrange in a number of ways. The absolute stereochemistries of the products have been ...
متن کاملA New Reporter Gene Technology: Opportunities and Perspectives
The paper summarizes the current status of the reporter gene technology and their basics. Reporter gene technology is widely used to monitor cellular events associated with gene expression and signal transduction. Based upon the splicing of transcriptional control elements to a variety of reporter genes, it “reports” the effects of a cascade of signaling events on gene expression inside cells. ...
متن کاملEmbedding of a 2D Graphene System in Non-Commutative Space
The BFT approach is used to formulate the electronic states in graphene through a non-commutative space in the presence of a constant magnetic field B for the first time. In this regard, we introduce a second class of constrained system, which is not gauge symmetric but by applying BFT method and extending phase space, the second class constraints converts to the first class constraints so th...
متن کاملEdge-outer graph embedding and the complexity of the DNA reporter strand problem
In 2009, Jonoska, Seeman and Wu showed that every graph admits a route for a DNA reporter strand, that is, a closed walk covering every edge either once or twice, in opposite directions if twice, and passing through each vertex in a particular way. This corresponds to showing that every graph has an edge-outer embedding, that is, an orientable embedding with some face that is incident with ever...
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ژورنال
عنوان ژورنال: Science
سال: 2006
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.314.5800.727d